Part:BBa_K1963000:Design
Hfq from E. coli K-12
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No illegal (RFC[10]) in the natural sequence.
Source
Amplified by PCR from genomic DNA.
References
Cech GM, Szalewska-Pałasz A, Kubiak K, Malabirade A, Grange W, Arluison V, Węgrzyn G. (2016) The Escherichia coli Hfq protein: an unattended DNA-transactions regulator. Front Mol Biosci 3: 36. doi:10.3389/fmolb.2016.00036.
Updegrove TB, Zhang A, Storz G. (2016) Hfq: the flexible RNA matchmaker.Curr Opin Microbiol 30:133-138. doi: 10.1016/j.mib.2016.02.003.
Pfeiffer V, Papenfort K, Lucchini S, Hinton JC, Vogel J. (2009) Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation. Nat Struct Mol Biol 16: 840-846. doi: 10.1038/nsmb.1631.
Chen S, Zhang A, Blyn LB, Storz G. (2004) MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli. J Bacteriol 186: 6689-6697.
Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols 8, 1694–1707. doi:10.1038/nprot.2013.105